Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

The source organisms that possess Taq polymerase are

Describe briefly the following

Polymerase Chain Reaction (PCR)

Write short note on the following

Downstream Processing

Name the enzyme used for the joining of DNA strand, whether it is ligase or endonuclease?

Write a short note on the role of restriction endonuclease in rDNA technology.

The restriction endonuclease is used to cut the DNA molecules at a specific site.

_____ of the DNA strands is the second step in PCR process.

The full form of PCR is

Which one among the following primer pairs would amplify the fragment of $\mathrm{DNA}$ given below?

$5^{\text{'}}-\mathrm{CTAGTCGTCGAT}-{\left(\mathrm{N}\right)}_{300}-\mathrm{GACTGAGCTGAGCTG}-3\text{'}$

$3^{\text{'}}·GATCAGCAGCTA-(\mathrm{N}{)}_{300}-\mathrm{CTGACTCGACTCGAC}-5^{\text{'}}$

The cell or organism from which the required gene for rDNA technique is taken is called recipient.

Choose the correct sequence of steps in $\mathrm{PCR}$

Identify the chief enzyme that is involved in $\mathrm{PCR}$ technique.

Which among the following does not help in early diagnosis?

The source of Taq polymerase used in PCR is a halophilic bacterium.

Microorganisms can be grown in the bioreactors by

The recombinant DNA technology is also popularly called as Genetic engineering or Medical engineering?

Describe gene amplification (PCR). 

PCR technique was developed by ____.